2016 · Paper V · 10 marksFrequently asked

What is plant tissue culture? Write applications of tissue culture in agriculture?

In short

Plant tissue culture is the aseptic growing of plant cells, tissues or organs on a controlled nutrient medium, relying on cells' ability to regenerate whole plants. In agriculture it yields virus-free planting material through meristem culture, multiplies elite varieties by micropropagation and aids germplasm conservation and breeding.

Model answer

Ghissu Editorial · Verified 4 Oct 2026

Introduction
Plant tissue culture is a key biotechnology for rapid, disease-free1 plant propagation, addressing challenges like pest infestations and slow crop multiplication. Initiatives such as the Clean Plant Programme highlight its growing importance in India’s agricultural development by providing quality planting material to farmers.
Understanding Plant Tissue Culture
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Limitations and Cost Factors

High costs and lack of standardized protocols for crops like mango and coconut limit scalability, especially in Andhra Pradesh. Additionally, infrastructure gaps and technical complexities restrict widespread adoption beyond high-value crops.

Applications of Tissue Culture in Agriculture
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Disease-Free Material and Elite Varieties

Meristem culture produces virus-free plants vital for sugarcane, potato, and banana, preventing pest and disease accumulation. Micropropagation supports rapid multiplication of superior cultivars, benefiting Andhra Pradesh horticulture by meeting demand for quality planting material.

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Germplasm Conservation and Breeding

Tissue culture aids genetic resource preservation and breeding of climate-smart varieties like drought-tolerant chickpea approved by the Central Sub-committee on Crop Standards2. It also facilitates doubled haploid production and genetic transformation, accelerating crop improvement.

Way Forward
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Expand Clean Plant Programme

Accelerate establishment of Clean Plant Centres in Andhra Pradesh to supply affordable, disease-free seedlings, enhancing horticultural productivity and farmer incomes.

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Promote Partnerships and Training

Foster public-private collaboration to scale tissue culture infrastructure and reduce costs. Implement farmer education and extension programs in Andhra Pradesh to improve awareness and adoption of tissue-cultured plants.

Conclusion
Plant tissue culture, combined with schemes like the Clean Plant Programme and supported by regional research and infrastructure, can significantly enhance agricultural productivity, sustainability, and resilience, contributing to the goals of SDG 2 and inclusive rural development.

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Hand-drawn diagram: Micropropagation in tissue culture. Micropropagation in tissue culture. Flow: Explant: shoot tip or node → Aseptic medium with nutrients, hormones → Totipotent cells regenerate shoots → Uniform, disease-free plantlets
Diagram: Micropropagation in tissue culture

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Human diseases; genetic engineering basics; biotechnology in agriculture; vaccines54 questions

Asked in 8 of 8 sittings since 2008

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Most tested: Vaccines and immunisation, alongside GM crops/biotech agriculture.

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